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For example, if an item is formulated and filtered just before a final sterilization, microbiological tests on the Bio-burden just after filtration will give some beneficial information with the willpower of the adequacy with the sterilization method. However, it will eventually present small, if any, facts pertaining towards the adequacy of the method with regard to minimizing endotoxin contamination. Since endotoxins end result from superior levels of microorganisms, and therefore are not removed by sterilizing or microbiological filters, the following reduction of the large microbiological amount will not be connected with an analogous reduction of superior endotoxin level.
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